Generator

Part:BBa_K4628048:Design

Designed by: Junjie Li   Group: iGEM23_ZJU-China   (2023-10-11)


50Spro-miniSOG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 517
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 517
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 517
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

MiniSOG is more phototoxic and it also has green-colored fluorescence for easy observation.


Source

The promoter was extracted and amplified from Agrobacterium tumefaciens str.C58 genome by PCR, separated from other nucleic acids by agarose gel electrophoresis. We constructed miniSOG fragments using gene synthesis services and overlap extension PCR. The terminator was obtained from iGEM provided plates. All of them ligated into a plasmid vector by Gibson assembly.

References

[1] Céline L ,Sylvain A ,Joaquim T , et al. Riboflavin-binding proteins for singlet oxygen production.[J]. Photochemical & photobiological sciences: Official journal of the European Photochemistry Association and the European Society for Photobiology,2022,21(9).